Detection of self-incompatible oilseed rape plants (Brassica napus L.) based on molecular markers for identification of the class I S haplotype
نویسندگان
چکیده
The selection of desirable genotypes with recessive characteristics, such as self-incompatible plants, is often difficult or even impossible and represents a crucial barrier in accelerating the breeding process. Molecular approaches and selection based on molecular markers can allow breeders to overcome this limitation. The use of self-incompatibility is an alternative in hybrid breeding of oilseed rape. Unfortunately, stable self-incompatibility is recessive and phenotype-based selection is very difficult and time-consuming. The development of reliable molecular markers for detecting desirable plants with functional self-incompatible genes is of great importance for breeders and allows selection at early stages of plant growth. Because most of these reliable molecular markers are based on discrimination of class I S-locus genes that are present in self-compatible plants, there is a need to use an internal control in order to detect possible PCR inhibition that gives false results during genotyping. In this study, 269 double haploid F2 oilseed rape plants obtained by microspore embryogenesis were used to verify the applicability of an improved PCR assay based on the detection of the class I SLG gene along with an internal control. Comparative analysis of the PCR genotyping results vs. S phenotype analysis confirmed the applicability of this molecular approach in hybrid breeding programs. This approach allows accurate detection of self-incompatible plants via a different amplification profile.
منابع مشابه
Transformation And Light Inducible Expression of cry1Ab Gene in Oilseed Rape (Brassica napus L.)
Rapeseed (Brassica napus L.) is the third most important oil crop in global productions. One of the major limiting factors for oilseed rape production is lepidopteran pests of the Brassicaceae family. Transgenic plants expressing Bacillus thuringiensis (Bt) genes are powerful tools in the integrated pest management of crop plants. In the present study, we used a synthetic Bt insecticidal crysta...
متن کاملAnalysis of Slg Gene – the Molecular Marker in Hybrid Breeding of Oil Seed Rape
Oil seed rape (Brassica napus L.) cultivars, donors of quality (SC) and self-incompatible (SI) lines have been analysed using identification of S-locus. In several Brassica napus cultivars one S-locus SLG gene was detected as dominant and the second S-locus as recessive. Amplification class II SLG gene screened recessive gene in all analysed samples (SC and SI). The DNA fragment of recessive ge...
متن کاملThe Use of Herbicides in Biotech Oilseed Rape Cultivation and in Generation of Transgenic Homozygous Plants of Winter Oilseed Rape (Brassica napus L.)
متن کامل
Interrelationships among grain yield and related characters of four oilseed rape (Brassica napus L.) cultivars under drought stress conditions
Four rapeseed cultivars (Hayola 401, Hayola 308, RGS and Option) were evaluated for some physiological traits under stress (50 % field capacity (FC) and non-stress (irrigated) conditions. The factorial set of treatments was arranged within a randomized complete block design with three replications. The collected data were analyzed using path and factor analyses. These is results showed that...
متن کاملEffect of tillage system, planting method and nitrogen fertilizer rate on agronomic charachteristics and seed yield of oilseed rape (Brassica napus L.) cv. Dalgan in Guilan, Iran
To study the effects of tillage system, planting method and of nitrogen fertilizer rate on the agronomic characteristics and seed yield of rapeseed (Brassica napus L.) Dalgan oilseed rape cultivar, as the second crop after rice, a split factorial experiment in complete randomized block design with three replications was carried out in two cropping seasons (2016-2018) at paddy fields of Rice Res...
متن کامل